Do you have a protocol or recommended antibodies to perform ICC staining on my totally differentiated ALI cultures?
Assess preclinical test compounds and biologics for their talents to modulate the immune system.
Evaluate the potential toxic effects of applicant therapeutics, like compact molecule compounds and biologics.
Using a P1000 pipette, gently triturate BLOs while in the apical chamber approximately three times even though circling the tip within the insert. Transfer contents to your 15 mL conical tube.
See info showing superior ALI society morphology and higher epithelial cell marker expression utilizing these recommended Transwell® inserts >
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Appraise the consequences of test compounds on hematopoietic stem cells with phenotypic and functional assessments.
Differentiation into adipocytes, osteoblasts, or chondrocytes with compatible MesenCult™ differentiation systems
Certainly, you’ll find the actions to perform an ICC staining on your epithelial cells cultured within the ALI During this protocol. Here is a listing of antibodies which can be used for the characterization of airway cultures:
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Of course, Stem cell you’ll locate the step-by-phase protocol for TEER measurement To judge the epithelial barrier integrity in ALI cultures here.
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